Kritika Rai, Kavitha Prasad, Parimala Sagar, Roopa S. Rao, C. H. Vanishri, M. Sri Gowri
Background: Podoplanin is a mucin-like transmembrane glycoprotein that been highly and specifically expressed in the lymphatic endothelial cells, but not in the endothelium of blood vessels. Studies have shown that podoplanin expression in tumor cells including those of squamous cell carcinomas and a relationship with the clinicopathological features. This raises a possibility that podoplanin might have a biological function in oral squamous cell carcinoma and its expression could be used as a biomarker for diagnosis and prognosis.
Methods: Formalin-fixed paraffin-embedded tissue blocks and histopathology reports of 50 cases of OSCC were used in this study. These were evaluated immunohistochemically for the expression of podoplanin using D2-40, a monoclonal antibody. The association between podoplanin expression and tumor site, size and degree of differentiation, lymph node metastasis, and prognosis was analyzed. Results: In this study, we observed that podoplanin was highly expressed in OSCC, but we found no significant association between podoplanin expression and clinicopathological characteristics and survival. Conclusion: The role of podoplanin as a marker for lymph node metastasis is questionable. To determine the role of podoplanin as a prognostic marker, further prospective studies are required with a longer follow-up period. Further studies using a combination of markers are required to predict tumor invasiveness and occult metastasis in OSCC.
Keywords: D2-40, immunohistochemistry, oral squamous cell carcinoma, podoplanin
How to cite this article: Rai K, Prasad K, Sagar P, Rao RS,
Vanishri CH, Gowri MS. Expression of podoplanin in
predicting the biological behavior of oral squamous cell
carcinoma – A clinicopathological correlation. J Adv Clin Res
Received: 20 November 2019;
Accepted: 22 December 2019
Suma Srinivasan, Amrita Samanta, B. Veerendra Kumar, M. G. Madhura, Sarita Yanduri
Microabscess refers to a small accumulation of polymorphs, lymphoid cells, or eosinophils in the epidermis and/or adnexal structures of the skin and epithelium of mucous membranes. Munro’s microabscess found in psoriasis vulgaris and psoriasiform oral lesions is an accumulation of neutrophils in stratum granulosum, stratum corneum, or stratum malpighi. Neutrophilic microabscess formation is also evident in various bacterial infections, Reiter’s disease, and fissure tongue. In mycosis fungoides, intraepidermal accumulation of monocytoid neoplastic lymphoid cells is referred to as Darier Pautrier microabscess. Lymphocytic accumulation in the epidermis is found in allergic contact dermatitis, lichen simplex chronicus, and pityriasis rosea. Microabscess of eosinophils is seen in pemphigus vegetans and incontinentia pigmenti, while dermatitis herpetiformis shows admixture of neutrophilic and eosinophilic microabscesses. Thus, microabscesses are characteristic of certain pathologies and are helpful in their diagnosis. Furthermore, insight into formation of these microabscesses may have a tale to tell regarding the disease process. Thus, this article attempts to review microabscesses and their significance in oral diseases.
Keywords: Epidermis, eosinophil, lymphocyte, microabscess, neutrophil
How to cite this article: Srinivasan S, Samanta A. Veerendra BV,
Madhura MG, Yanduri S. The tale of microabscesses: A review.
J Adv Clin Res Insights 2019;6:178-182.
Received: 28 November 2019;
Accepted: 29 December 2019
Hortencia Recio, Leili de Leon, Juan Carlos Sebastian Sanchez
Background: Provisional fixed restoration is an essential component in prosthodontics treatment. These materials may experience color change for surface roughness. To reduce it, a polishing technique is required with a time setting specified.
Aims and Objectives: The aim of this study was to evaluate the effect of different polishing time on the color stability of provisional materials.
Materials and Methods: One hundred and forty-four resin blocks were prepared, divided into Group A, PMMA acrylic resin and Group B, bis-acryl resin. They were polished with goat hairbrush and diamond paste. Twenty-four hours stored in distilled water and 12th day on the staining solution coffee and wine. They were measured with a spectrophotometer, analyzed, and processed with the CieLab system, to calculate color difference ΔE.
Results: On the 3rd day of the immersion period, the lowest mean value was showed with 1 min polishing on Group A, showing to be statistically significant, P = 0.011. In Group B, the lowest mean value was showed with 1 min 30 s. At the 7th and 12th days immersion period, the lowest mean value was showed with 30 s polishing among the groups.
Conclusion: Polishing 30 s showed the lowest ΔE values on PMMA and bis-acryl resin. Mean color differences were above the clinical acceptability threshold ΔE > 3.3. Regarding the staining agent, the highest ΔE values were observed in coffee.
Keywords: Acrylic resin, bis-acryl composite resin, color, polishing
How to cite this article: Recio H, de Leon L, Sanchez JCS. Effect of different polishing time on the color stability of provisional materials: An In vitro study. J Adv Clin Res Insights 2019;6:138-143.
Received: 25 August 2019;
Accepted: 26 September 2019
S. Sangeetha, Kiran Mitra, Umesh Yadalam, Sarita Joshi Narayan
It has been a topic of debate among dental care professionals, if trauma from occlusion is linked with periodontal disease or not. There are several schools of thought related to if trauma from occlusion is an etiological factor or cofactor for the occurrence of periodontal diseases. The present review article discusses historical background, etiological factors, classification, relevant terminologies, tissue response, signs and symptoms, advanced diagnostic methods, and treatment.
Keywords: Occlusal forces, periodontium, traumatic occlusion
How to cite this article: Sangeetha S, Mitra K, Yadalam U, Narayan SJ. Current concepts of trauma from occlusion - A review. J Adv Clin Res Insights 2019;6:14-19.
Received: 02 January 2019 ;
Accepted: 12 February 2019
Sushant A. Pai, Shruti R. Poojari, Keerthi Ramachandra, Sajeev Bhaskaran, Mangala Jyothi
The terminology “temporomandibular disorder” (TMD) covers a group of conditions. A lot of attempts have been made to categorize TMD, but all have shortfalls. Some classify by frequency of presentation, some by etiology, and some by anatomy. However, there is substantial overlap in classification system as they are not clinically suitable. Therefore, not even one system persuades all the criteria. TMD is a wide-ranging collection of clinical problems that involve the muscle of mastication, the temporomandibular joint, surrounding bone and soft tissue components, or at times combinations of all of them. Any factor that affects one part of the system is likely to have impact on the other parts also, so it is essential to avoid blind side when we have to consider likely signs and symptoms of a TMD. About 20-30% of the adult populations are affected to some degree; it is predominately a condition of young and middle-aged adults, rather than of children or the elderly, and is approximately twice as common in women as in men. TMD is the second most common non-dental origin orofacial pain, but at the same time, the recurrence of other symptoms such as earache, headache, neuralgia, and tooth pain, which can be related to the TMD or be present as secondary findings to be assessed in the differential diagnosis process.
Keywords: Epidemiology, temporomandibular disorders, treatment
How to cite this article: Pai SA, Poojari SR, Ramachandra K, Bhaskaran S, Jyothi M. Temporomandibular Joint Disorders - Part I. J Adv Clin Res Insights 2019;6:6-10.
Received: 14 December 2018 ;
Accepted: 22 January 2019